Table 1.
PCRs, RT‐PCRs and real‐time PCRs used in this study
| Pathogen | PCR‐type | Primer/probe sequences | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
| CRCoV | Real‐time RT‐PCR |
Primer‐F 5'‐ACGTGGTGTTCCTGTTGTTATAGG‐3' Primer‐R 5'‐AACATCTTTAATAAGGCGACGTAACAT‐3' Probe: FAM‐5'‐CCACTAAATTTTATGGCGGCTGGGATG‐3'‐TAMRA |
60 | Spiss et al. 2012 |
| CPiV | RT‐PCR |
Primer‐F: 5'‐AGTTTGGGCAATTTTTCGTCC‐3' Primer‐R: 5'‐TGCAGGAGATATCTCGGGTTG‐3' |
55 | Erles et al. 2004 (modified) |
| CAV‐1/CAV‐2 | PCR |
Primer‐F: 5'‐GCCACTACTCTCCTGTTGAT‐3' Primer‐R: 5'‐GAAGAAGAAGTCCGAGACAC‐3' |
60 | Nell et al. 2000 |
| CDV | RT‐PCR |
Primer‐F: 5'‐ACAGGATTGCTGAGGACCTAT‐3' Primer‐R: 5'‐CAAGATAACCATGTACGGTGC‐3' |
60 | Frisk et al. 1999 |
| CDV | Real‐time RT‐PCR |
Primer‐F: 5'‐AGCTAGTTTCATCTTAACTATCAAATT −3' Primer‐R: 5'‐TTAACTCTCCAGAAAACTCATGC‐3' Probe: FAM‐5'‐ACCCAAGAGCCGGATACATAGTTTCAATGC‐3'‐TAMRA |
48 | Elia et al. 2006 |
| Influenza A | Real‐time RT‐PCR |
FLUAM‐1F: 5'‐AAGACCAATCCTGTCACCTCTGA −3' FLUAM‐1R: 5'‐CAAAGCGTCTACGCTGCAGTCC ‐3' FLUAM‐1P: 5'‐FAM‐ TTTGTGTTCACGCTCACCGT‐TAMRA‐3' |
60 |
http://www.who.int/csr/resources/ publications/swineflu/WHO_Diagnostic _RecommendationsH1N1_20090521 .pdf (modified) |
RT‐PCR Reverse transcription PCR, CRCoV Canine respiratory coronavirus, CPiV Canine parainfluenza virus, CAV‐1/2 Canine adenovirus type‐1/2, CDV Canine distemper virus