Table 4.

Detection of anti‐CRCoV antibody titres in paired serum samples (acute; convalescent) from dogs of group A and B

	Dogs	Acute	2 to 3 weeks later	Titre increase	CRCoV (qPCR)
Group A	1	<1:20	1:2560	>128‐fold	Positive
	2	<1:20	1:2560	>128‐fold	Positive
	3	1:40	1:1280	32‐fold	Negative
	4	<1:20	1:320	>16‐fold	Negative
	5	1:80	1:640	Eightfold	Negative
	6	1:640	1:1280	Twofold	Negative
	7	1:320	1:640	Twofold	Negative
	8	1:160	1:320	Twofold	Negative
	9	1:320	1:320	0	Negative
	10	1:320	1:320	0	Negative
	11	<1:20	<1:20	0	Negative
	12	1:160	1:160	0	Negative
	13	1:640	1:640	0	Negative
	14	1:1280	1:1280	0	Negative
	15	1:2560	1:2560	0	Negative
	16	<1:20	<1:20	0	Negative
	17	1:640	1:640	0	Negative
Group B	1	<1:20	1:2560	>128‐fold	Positive
	2	<1:20	1:1280	>64‐fold	Positive
	3	<1:20	1:1280	>64‐fold	Positive
	4	<1:20	1:1280	>64‐fold	Positive
	5	1:80	1:1280	16‐fold	Positive
	6	1:80	1:1280	16‐fold	Positive
	7	<1:20	1:2560	>128‐fold	Negative
	8	1:80	1:2560	32‐fold	Negative
	9	1:320	1:1280	Fourfold	Negative
	10	1:640	1:2560	Fourfold	Negative
	11	1:640	1:1280	Twofold	Negative
	12	1:2560	1:2560	0	Negative
	13	≥⃒1:5120	1:2560	0	Negative
	14	n.a.	n.a.	n.a.	Positive
	15	n.a.	n.a.	n.a.	Negative

CRCoV Canine respiratory coronavirus, qPCR Quantitative PCR, n.a. Not available, Group A Privately owned diseased dogs, Group B Kennelled diseased dogs