Figure 6.

Map‐based cloning of TIP3. (a) Genetic linkage map for the primary location of the TIP3 locus on chromosome 3. (b) The TIP3 locus was fine‐mapped to a 21.4 kb region. (c) Prediction of three putative open reading frames (ORFs) in the fine mapping genomic region. (d) Gene schematic of the TIP3 (ORF3). Black boxes represent exons; the gray lines between exons represent introns. (e–h) The spikelet phenotype of wild‐type, tip3,TIP3‐complemented line and tip3‐2 (homozygous line knocked out by CRISPR/Cas9), respectively. Bars represent 500 μm. (i–l) The pollen grains were stained with 1% I₂‐KI solution at stage 13 from the wild‐type, tip3,TIP3‐complemented line and tip3‐2, respectively. Bars represent 5 μm.