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. 2019 Jul 30;597(17):4521–4531. doi: 10.1113/JP277985

Figure 3. End‐diastolic force not SL determines length‐dependent activation.

Figure 3

During the work loop protocol cells are stretched to different preload (end‐diastolic force; EDF), while SL is recorded. A, the EDF to SL relationship shows that HET cardiomyocytes require much less force to be stretched, which holds true at different pacing frequencies (N = 6, n = 18 WT; N = 6, n = 17 HET). B, as developed force is measured during the subsequent contraction, the developed force to end‐diastolic SL relationship can be measured. This relationship is a measure of length‐dependent activation. This is severely blunted in HET cardiomyocytes (N = 6, n = 17 WT; N = 6 n = 9 HET). C, however, if EDF is plotted against subsequent developed force, only a small non‐significant decrease is seen in HET cardiomyocytes compared with WT (N = 6, n = 21 WT; N = 6, n = 10 HET).