FIGURE 3.

Phylogenetic trees constructed from partial sequence analysis of the 16S subunit 2 ribosomal ribonucleic acid gene of Glossina brevipalpis (a) and Glossina austeni (b) from Eswatini (Esw), Mozambique (Moz E; Moz W) and South Africa [Ndumu (NDU), Tembe (TEM), Kosi Bay (KOS), Mbazana (MBA), Mkuzi (MK), Lower Mkuzi (UMB), Phinda (PI), False Bay Park (MFP), Hluhluwe-iMfolozi Park north (HIP), Hluhluwe-iMfolozi Park south (HP), Boomerang (MFK), St Lucia (ST)]. Colony (Col) flies of both species were used as out-groups. Red and blue groupings indicate the South Africa-southern Mozambique and Eswatini haplotypes, respectively. Arrows indicate specimens carrying contrasting haplotypes from their group.