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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Biochemistry. 2019 Oct 7;58(41):4260–4268. doi: 10.1021/acs.biochem.9b00732

Figure 2.

Figure 2.

Averages of five pre-steady-state traces for 17X-PTDH at 45 °C (A) and 5 °C (B). Traces were obtained by mixing a solution of 17X-PTDH (10 μM in Panel A; 150 μM in Panel B) with a solution of 10 mM 1H- or 2H-PT, 10 mM NAD+ in 100 mM MOPS, pH 7.25. Data is fit (dashed line) to the zeroth-order rate law. Absorbance values corresponding to a single turnover are 0.062 in Panel A and 0.93 in Panel B.