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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Biochemistry. 2019 Oct 7;58(41):4260–4268. doi: 10.1021/acs.biochem.9b00732

Table 3.

Isotope effects on tunneling-related parameters derived from Arrhenius plots with Thr104 mutantsa

Variant AH/AD ΔEa (kcal/mol)
17X-PTDH 1.6 (0.1) 0.21 (0.05)
T104S 0.66 (0.07) 0.75 (0.06)
T104A 0.45 (0.05) 0.96 (0.06)
T104G 0.60 (0.05) 0.77 (0.05)
a

All values were derived from fits of lnDkcat against 1/T according to equation 2.