Table 3.
Isotope effects on tunneling-related parameters derived from Arrhenius plots with Thr104 mutantsa
| Variant | AH/AD | ΔEa (kcal/mol) |
|---|---|---|
| 17X-PTDH | 1.6 (0.1) | 0.21 (0.05) |
| T104S | 0.66 (0.07) | 0.75 (0.06) |
| T104A | 0.45 (0.05) | 0.96 (0.06) |
| T104G | 0.60 (0.05) | 0.77 (0.05) |
All values were derived from fits of lnDkcat against 1/T according to equation 2.