Phenotypic characterization of vitamin B6 enhanced rice lines under stress conditions.
(a) Pictures of plantlets as indicated from the T3 generation grown in hydroponic culture 15 days after application of salt stress (150 mm NaCl). The scale bar represents 3 cm.
(b) Leaf length, root length, leaf fresh weight (FW) and root fresh weight of 35S lines in (a) compared to wild‐type (TP309) and the empty vector transgenic control (p1300). Average ± SD of 12 biological replicates. Tukey's multiple comparison test (P < 0.05).
(c) Lesion length in rice leaves inoculated with Xanthomonas oryzae. Lesion length in leaves of 35S‐12a, T3 generation, 15 days after inoculation with PX071 (Leach et al., 1992) and BAI3 (Gonzalez et al., 2007a) Xanthomonas oryzae pv. oryzae strains compared to wild‐type (TP309) and the empty vector transgenic control (p1300). Inoculation with water was performed as a negative control. Average ± SD of four replicates for water and 8–12 replicates for bacterial inoculation (PX071‐TP309 (n =8), PX071‐p1300 (n = 7), PX071‐35S‐12a (n = 8), BAI3‐TP309 (n = 12), BAI3‐p1300 (n = 9), BAI3‐35S‐12a (n = 8)). Student's t‐test (p1300 versus transgenic line), **P < 0.01.