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. 2019 Jul 11;99(6):1047–1065. doi: 10.1111/tpj.14379

Figure 7.

Figure 7

PDX transgene expression and protein accumulation in leaves, unpolished seeds, and polished seeds.

(a) AtPDX1.1 and AtPDX2 transcript expression levels in 35S transgenic rice lines (T2 generation) compared with wild‐type (TP309) and the empty vector control (p1300). Average ± SD of three biological replicates. Tukey's multiple comparison test (P < 0.05) for each tissue.

(b) Western blot analysis of AtPDX1.1 and AtPDX2 protein abundance (upper panel) and Ponceau staining of the nitrocellulose membrane (lower panel) in control and transgenic rice lines. Here, 50 μg of total rice proteins and 30 μg of total Arabidopsis leaf proteins were probed with peptide antibodies specific to AtPDX1.1 (Raschke et al., 2011) or AtPDX2 (Tambasco‐Studart et al., 2007). M, protein molecular‐weight marker; L, leaves; US, unpolished seeds; PS, polished seeds; At, Arabidopsis thaliana.