Fig. 3.

Knocking down PES1 increases pancreatic cancer cell sensitivity to BET inhibitors. a, PANC-1 cells were transfected with indicated plasmids for 72 h. Cells were treated the different types of small inhibitors for 48 h, the IC50 values was analyzed and IC50 ratio of Control vs shPES1m and Control vs Flag-PES1 were determined and shown in heatmap. shPES1m indicated that mixed shRNA (shPES1#1 and shPES1#2). b and b, pancreatic cancer cells were infected with indicated plasmids for 72 h. Then, cells were treated with or without JQ1 (5uM) for MTS assay and colony formation assay. Data presented as Means ± SD (n = 3). **, P < 0.01; ***, P < 0.001. d, PANC-1 cells were infected with indicated plasmids for 72 h. Then, cells were treated with or without JQ1 (5uM) for 48 h. Cells were subjected to Annexin-V/Propidium Iodide (PI) assay. Data presented as Means ± SD (n = 3). ***, P < 0.001. e-h, PANC-1 cells were infected with indicated shRNA for 72 h. Cell were subcutaneously injected into the nude mice. These mice were treated with or without JQ1 for 27 days. These tumors were harvested for photograph (e), weight (f), tumor growth curve mapping and caspase 3 analysis by IHC (h). Data presented as Means ± SD (n = 5 for e, f and g, n = 3 for h). *, P < 0.05; **, P < 0.01; ***, P < 0.001