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. 2019 Nov 13;12:69. doi: 10.1186/s13072-019-0312-7

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Low folate and MTX induced craniorachischisis in C57BL/6 mouse embryos at 13.5 day. b Control vs MTX treatment in mESC. The total RNA was analyzed using RNA-seq. A total of DEGs showing differential expression were identified in the APN-treatment group (P < 0.01). c Unsupervised hierarchical clustering of MTX-treated cells. d DEGs have a statistically significant change with RNA-seq analysis. e NTC-related genes Cdx2, Nestin, Pax6 and Gta4 mRNA extracted from mESCs treated with MTX was assessed by RT-qPCR. MTX 1 μM, 12 h. GAPDH was used as control. Data are shown as mean (n = 3). *P < 0.05, by Student’s t test