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. 2019 Nov 13;12:69. doi: 10.1186/s13072-019-0312-7

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — a MTX induced Myelomeningocele in C57BL/6 mouse embryos at 13.5 day. b Folate content in brain serum of normal and MTX-induced mouse NTDs was harvested at E13.5, and analyzed by Elisa. Data are mean ± S.D. (n = 3). *P < 0.05, by Student’s t test. c Cranial neural tissue of normal and MTX-induced mouse NTDs was harvested at E 13.5, and analyzed by western blot. Aliquots of total lysates were immunoblotted to indicate antibody. d Neural precursor marker genes cdx2, nestin, pax6, gata4 and mdm2 extracted mRNA in cranial neural tissue of MTX-induced mouse NTDs was measured by RT-qPCR. Data are mean ± S.D (n = 3). *P < 0.05, by Student’s t test