Fig. 7.

a Elisa assay detected folate content in brain tissue of normal fetus and fetus with NTDs. b Detection of H2AK119ub1 in brain tissues from normal fetus and low-folate NTDs with low folate maternal serum. H2A was used as a loading control. Data are mean ± SD. (n = 3),*P < 0.05, by Student’s t test. c Quantification of the western blotting H2AK119ub1 signal intensity shown in scatter plot. Column normals: n = 8; Column NTDs: n = 8; *p < 0.05, by Student’s t test. NTDs vs. normal brains. d Pearson R- and P value for normalized H2AK119ub1 levels versus low folate content in low-folate NTDs fetuses (n = 8). Data are presented as mean ± SEM. ***P = 0.002. e The mRNA expression of NTC-related genes CDX2, NESTIN, PAX6 and GATA4 in the NTDs fetuses brain, determined by Nanostring. Data are mean ± SD (n = 10),*P < 0.05, by Student’s t test. f Pearson’s correlation analysis between H2AK119ub1 expression and NTC-related genes CDX2, NESTIN, PAX6 and GATA4