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. 2019 Nov 12;17:371. doi: 10.1186/s12967-019-2125-7

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Automatic workflow diagram. Workflow showing the automatic procedures performed by the Freedom EVO 150 for blood stratification and PBMC collection. The blood stratification procedure consists of four necessary steps: (1) density media dispensation; (2) blood sample dilution; (3) diluted blood stratification, and (4) tube recovery for centrifugation (step highlighted in yellow). PBMC collection was performed after centrifugation and consisted of two steps: (1) the removal of 1.5 mL of supernatant and (2) the collection of PBMCs by means of aspirating 3.4 mL of the cellular suspension and subsequent distribution in 15 mL conical tubes containing DPBS + 2% FBS