Figure 10.

Acridine Orange/Ethidium Bromide and Hoechst Staining of Ethyl Acetate Extract Nannochloropsis hexane. To differentiate live and dead cells based on their morphological features, treated and untreated cells were stained with Acridine orange/Ethidium bromide, Hoechst and visualised using fluorescent microscopy. A, Acridine orange/Ethidium bromide staining of untreated cells displayed green nuclei with an intact nuclear architecture; B, Acridine orange/Ethidium bromide staining of Ethyl acetate extract Nannochloropsis hexane fraction treated cells displayed green and red nuclei with cell shrinkage, nuclear condensation; C, Hoechst staining of untreated cells; D, Hoechst staining of Ethyl acetate extract Nannochloropsis hexane fraction treated cells. This result strongly demonstrates that the IC₅₀ concentration (175 µg/ml) of extracted fraction hold the ability to induce apoptosis in the Human Non-small lung cancer cell line (A549)