Table 1.
Gene | Primer pair sequences (5–3) | Amplification | |
---|---|---|---|
length (bp) | |||
DNA level | IGF1R-P1 | 1F: GACTATGGCACCAGTGTTTGT | 171 |
|
1R: CCTTGAGGCTATCGCTGTATT |
|
|
BTF3 | 2F: CAAGAAGACTCATTCCTT | 109 | |
|
|
2R: CACAAGCACATTATTCAC |
|
mRNA level | IGF1R-P2 | 3F: CTCAAGGACGGAGTCTTCACC | 106 |
|
3R: CATTGGACAGGCCCTGATAC |
|
|
-actin | 4F: CTTCCTGGGCATGGAATCCTG | 103 | |
4R: CAGCACCGTGTTGGCGTAG |
Note: F – forward primer; R – reverse primer.