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. 2019 Sep 18;62(2):547–555. doi: 10.5194/aab-62-547-2019

Table 1.

Primer sequences used for the amplifications of the bovine FADS2 exons (1, 3, and 12).

Region Primer sequences (5–3) Product Annealing
    length temp. (C)
Exon 1 F1: GGAGGAGAAGACAAAAGCCGA 437 60
 
R1:TGAGCGCCGTAGACACTTTT
 
 
Exon 3 F3: TCCCAGATCACCGAGGACTT 292 60
 
R3: TTCAGAGCGTTGGCACCTAG
 
 
Exon 12 F12: CGGGCAACTGGTCCCTTTAT 389 60
  R12: GTCCCATGACCAAGTGCCTC