Table 1.
Region | Primer sequences (5–3) | Product | Annealing |
---|---|---|---|
length | temp. (C) | ||
Exon 1 | F1: GGAGGAGAAGACAAAAGCCGA | 437 | 60 |
|
R1:TGAGCGCCGTAGACACTTTT |
|
|
Exon 3 | F3: TCCCAGATCACCGAGGACTT | 292 | 60 |
|
R3: TTCAGAGCGTTGGCACCTAG |
|
|
Exon 12 | F12: CGGGCAACTGGTCCCTTTAT | 389 | 60 |
R12: GTCCCATGACCAAGTGCCTC |