Fig. 4.

The heatmap and clustering analysis for metabolic pathways. a Heatmap of K-means clustering have identified groups of proteins with various expression in different treatment groups. The heat map color codes reflect a change. K-means clustering was performed for 3963 protein-coding genes across all studied groups (adjusted p-value < 0.05). The model-based optimal number of K = 14 was determined. b Fourteen Pie charts present the KEGG enrichment analysis for metabolic pathways and correspond to 14 clusters