Table 1.

Primer detail.

Target Gene	Primer Sequence (5’->3’)	Amplicon size (bp)	Annealing Temp (°C)	GeneBank/Accession Number
Ocln	Sense : TGGCGGAGAGATGCACGTTCG Antisense : ACCGAAGCCGCTGCCGTAAG	247	55.2	NM_031329.2
Cldn5	Sense : CGTGACGGCGCAGACGACTT Antisense : TGCACTGAGCGCCGGTCAAG	194	57.8	NM_031701.2
Jam3	Sense : TCAAGGAGACCTGGCCGGTCG Antisense : GCCGAGGATAGCCCTCGCTCT	254	62.9	NM_001004269.1
ZO-1	Sense : GGCTACAGCCCGCGGCATTT Antisense : CCATGGGGCCCGCACATCAC	213	56.7	NM_001106266.1
ZO-2	Sense:TCCCGGGACTACAGCCGTGG Antisense : GCCGGGACCTAGACTCGGGG	208	59.1	NM_053773.1
AF-6	Sense : GCGATGGGCGAGGCTGAAACA Antisense : ATCAGGTGTCTCCCGCTCCACAG	180	62.9	NM_001007754.1
β actin	Sense : TCCACCCGCGAGTACAACCTTC Antisense : GGGCCACACGCAGCTCATTGTA	358	63.9	NM_031144.2
BDNF	Sense:TTGCCACAGCCCCAGGTGTGA Antisense : ACGCCTGTCACTGCGCCCTA	134	62.0	NM_012513.3
TRKB	Sense:ACTCTGCCAGCCCTCTCCACC Antisense: CGGCTTGAGCTGGCTGTTGGT	152	56.5	NM_012731.2
Ras	Sense:TGTGCTGTCCTGACACCAGGCT Antisense : TGGACTGGACTGGCTCCAGCA	118	55.7	NM_001105753.1
Raf	Sense:TGGGATTGGCTCGGGCTCCT Antisense :TGCGCAAAACAGCCACCTCGT	137	55.7	NM_012639.2
Mek 1	Sense:GCTGGATGAGCAGCAGCGGA Antisense : CCACCATTGCCAGCCCCCAG	117	56.5	D14591.1
Mek 2	Sense:CATGGGGCTGTCGCTGGTGG Antisense : TGGGGCTCTCCATCTGCCCC	120	56.5	D14591.1
Erk 1	Sense:ACCGGGACCTGAAGCCCTCC Antisense:GGCTCGGTACCAGCGTGTGG	146	62.9	NM_017347.2
Erk 2	Sense:CTCCTCGTTCCCGATCGCCG Antisense:GGGCTCGACGCTTCGCGTTA	100	58.6	NM_053842.1
CREB	Sense:TGATGCACCAGGGGTGCCAA Antisense:TGCTCCTCCCTGGGTAATGGC	142	55.7	NM_134443.1