Figure 6.

Metal-enhanced solution assays. One approach to the use of MEF in solution-based assays is to use oligonucleotide-modified silver particles in solution together with fluorophore-labeled complementary oligonucleotides. The schematic illustrates the preparation, displacement by thiolate oligonucleotides, and hybridization with fluorescein-labeled complementary oligonucleotides. The silver nanoparticles were protected with a tiopronin monolayer. Silver particles are shown as gray spheres and fluorophore molecules as black ovals. (Figure adapted from [31] with permission.)