Fig. 5.

Immunohistochemistry (IHC) analysis and quantification for EGFR, TGF-β and pMLC2 markers in whole section samples. A) Each marker (EGFR, TGF-β or pMLC2) is represented using the IHC staining (left), its H-score (middle) representation and rainbow map (right) using digital pathology approach. Scale bar: 1000 μm. B) Representative images for EGFR, TGF-β or pMLC2 corresponding to the TB and IF areas highlighted in A. Scale bar: 100 μm. Graphs type: “before and after” using lines and dots to represent TB and IF matched for each patient. Student's Paired t-test was used: *p < 0.05, **p < 0.01. Abbreviations: TB, tumour body; IF, invasive front.