Fig. 6.

Analysis of EGFR, TGFB1 and related genes expression in tissues from HCC patients shows prognosis significance. A) E-CADHERIN (CDH1), RHOC (RHOC) and CDC42 (CDC42) expression analysed by qRT-PCR where relative expression of each HCC tumour tissue versus its respective surrounding tissue was calculated and represented as % (cut-off ≥ 1). Representation of EGFR and TGFB1 expression on a heatmap of the same cohort of HCC patients is on the right. Kaplan-Meier estimation of 5 years survival in The Cancer Genome Atlas (TCGA) of HCC patients according to EGFR expression, TGFB1 expression and the combination of both (B), as well as according to EGFR expression combined with RHOC and CDC42 expression (C) (N = 327 HCC patients). Kaplan–Meier method using the log-rank test was used: *p < 0.05 and **p < 0.01.