Illustration of a typical mitochondrial stress test only using injection ports A-C. Oligomycin is released from port A, FCCP from port B and rotenone and antimycin A from port C. OCR data can be manipulated to determine mitochondrial parameters such as basal respiration (BR), ATP-linked respiration (ALR), proton leak (PL), maximum respiration (MR), spare respiratory capacity (SCR) and non-mitochondrial respiration (NMR).