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. 2019 Dec;61:104595. doi: 10.1016/j.tiv.2019.104595

Fig. 2.

Fig. 2

Transporter function and inhibition in HepaRG cells. HepaRG cells were incubated with (A) CMFDA (5 μM) and Hoechst (1:5000) only for 30 min; (B) CMFDA, Hoechst and the MRP inhibitor MK571 (30 μM) for 30 min; (C) CMFDA, Hoechst and the BSEP inhibitor bosentan (50 μM) for 30 min. Maximum intensity projection images with Apotome were taken using a Zeiss microscope using 40 x oil objective. Arrow indicates CMFDA within bile canaliculi whereas the circles indicate CMFDA retained within the cell cytoplasm. Scale bar = 20 μm.