Figure 2.

Effect of blood collection and handling on the coagulant properties of human plasma. Platelet-poor plasma (single centrifugation to remove platelets) collected in a siliconized glass tube (a) or plastic tube (b); (c) platelet-depleted plasma (double centrifugation) prepared from blood collected in a plastic tube. For the fibrin generation test (FGT, left), the prepared plasma samples (containing endogenous EVs) were recalcified at t = 0 as described in Methods. For the thrombin generation test (TGT, right), EVs were isolated from the prepared plasma samples and reconstituted in EV-depleted normal plasma as described in Methods, and thrombin generation was initiated by recalcification at t = 0. Please notice that the time scale (X-axis) differs for FGT and TGT. Representative data from one volunteer are shown. In total, six independent experiments were performed.