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. Author manuscript; available in PMC: 2019 Nov 13.
Published in final edited form as: Stem Cell Res. 2019 Aug 7;40:101529. doi: 10.1016/j.scr.2019.101529

Figure 1. Characterization of cardiomyocytes differentiated from healthy (CCm), FRDA (PCm) and ZFN-edited (ECm) iPSCs.

Figure 1

(A) Timeline and major steps of iPSC differentiation into beating cardiomyocytes. See Supplemental Methods for details. (B-D) Expression of cardiac markers analyzed by immunostaining. Nuclei were stained by DAPI and merged images are shown. (B) ACTC1 (Actin, Alpha, Cardiac Muscle 1); (C) TNNT2 (Troponin T2, Cardiac Type); (D) NKX2.5 (NK2 Homeobox 5). (E) Electron microscopy images of Cm ultrastructure (PCm top panel and CCm bottom panel); M - mitochondria, D – desmosomes, and Z – Z-bands.