Skip to main content
. 2019 Nov 1;15(11):e1008463. doi: 10.1371/journal.pgen.1008463

Fig 4. NPM and NPM-MLF1 bind to chromatin and facilitate the recruitment of the CHD4/NuRD complex.

Fig 4

A-C) ChIP-qPCR assays; chromatin of MOCK, NPM, NPM-MLF1, shScramble (shSc) and ShNPM (shNPM300) cells was immunoprecipitated with HA, CHD4 antibodies or isotype-matched immunoglobulins (IgG); SPARC TSS: -120bp to +8bp; CARD9 TSS: -99bp to -37bp; MS4A3 TSS: -6bp to +74bp; fold enrichments were relative to the neuronal Pax6 regulatory element, used as internal control; *: p ≤0.05 obtained by unpaired Student’s t test; N.S: Non-significant enrichment compared to corresponding IgG; D) Western blots of whole cell extracts; the antibodies used are indicated on the right side of the panels; α-Tubulin was used as loading control.