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. 2019 Nov 1;15(11):e1008463. doi: 10.1371/journal.pgen.1008463

Fig 5. NPM and NPM-MLF1 influence the chromatin organization of SPARC, CARD9 and MS4A3 genes.

Fig 5

A-D) ChIP-qPCR assays; chromatin of MOCK, NPM-MLF1, shScramble and shNPM300 (ShNPM) cells was immunoprecipitated with H3ac (K9 and K14 acetylation), H3K4me3, H3K27me3, H3K79me3, H3K36me3, Pol II, Ser2-phosphorylated Pol II C-terminal domain (PCTD), TFIID antibodies or isotype-matched immunoglobulins (IgG); SPARC TSS: -120bp to +8bp; CARD9 TSS: -99bp to -37bp; MS4A3 TSS: -6bp to +74bp; fold enrichments were relative to the neuronal Pax6 regulatory element, used as internal control; *: p ≤0.05 obtained by unpaired Student’s t test; N.S: Non-significant enrichment compared to corresponding IgG.