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. Author manuscript; available in PMC: 2020 Apr 8.
Published in final edited form as: ACS Biomater Sci Eng. 2019 Mar 8;5(4):1937–1943. doi: 10.1021/acsbiomaterials.8b01356

Figure 2. Cell Usage Schematic.

Figure 2.

Of the patient samples collected, two (referred to as samples 1-4-17 and 7-23-17) were cultured and subsequently characterized or treated with chemotherapy. Isolated cells from Sample 1-4-17 were placed directly into organoids and grown for 3 weeks at the end of which they were characterized using IHC and H&E. Sample 7-23-17 cells were isolated and placed directly into organoids grown for either 3 or 6 weeks, or grown on TCP. Organoids grown for 3 weeks were either characterized using IHC and H&E or used for drug treatment. Organoids grown for 6 weeks were used for characterization. Sample 7-23-17 cells grown on TCP were initially placed in either 96-well or 6-well TCP plates. Those in the 96-well plate were used for drug treatment. Those in the 6-well plate were passaged at approximately 3 weeks and those cells were made into organoids. The organoids from passaged TCP wells were grown for 3 weeks and characterized.