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. 2019 Nov 12;8:e48309. doi: 10.7554/eLife.48309

Figure 7. P. falciparum drives the expansion of human FcRL5+ T-bet+ B-cells that secrete anti-PS antibodies in vitro.

(A) Percentage of T-bet+FcRL5+ B-cells that expanded from the PBMCs of a healthy naïve donor after in-vitro exposure to either uninfected erythrocyte lysate (uLysate) or P. -falciparum-infected erythrocyte lysate (iLysate). (B) ELISPOT of enriched populations for either FcRL5 (gray bars) or CD27 (black bars) from PBMCs of healthy naïve US donors after in-vitro exposure to P.-falciparum-infected erythrocyte lysate (iLysate) (N = 3). ASC, antibody-secreting cells. Significance assessed by unpaired Student's t test. **p≤0.01, ***p≤0.001.

Figure 7—source data 1. Source data for Figure 7.
elife-48309-fig7-data1.zip (16.1KB, zip)
DOI: 10.7554/eLife.48309.041

Figure 7.

Figure 7—figure supplement 1. Total antibody-secreting cells among the CD27+- and FcLR5+-enriched PBMC.

Figure 7—figure supplement 1.

Total number of anti-IgM spots of antibody-secreting cells (ASCs) from either CD27+- or FcRL5+-enriched PBMC that were stimulated with P.-falciparum-infected erythrocyte lysate. Significant assessed by unpaired Student's t test, ****p<0.0001.
Figure 7—figure supplement 2. Stimulation with P. falciparum Histidine Rich Protein II (HRPII) does not stimulate the expansion of atypical MBCs in vitro.

Figure 7—figure supplement 2.

Stimulation in vitro of naïve PBMC from healthy US donors (n = 3) with medium, P. falciparum HRPII, uninfected erythrocyte lysate (uLysate) or P.-falciparum-infected erythrocyte lysate (iLysate). Significance was assessed by one-way Anova. *p<0.05, **p<0.01.