Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | TMC1 | NCBI ID: 13409 |
||
| Gene (Mus musculus) | TMC2 | NCBI ID:192140 | ||
| Gene (Mus musculus) | Lhfpl5 | NCBI ID: 328789 |
||
| Strain, strain background (Mus musculus) | C57BL6 | Vitalriver | ||
| Genetic reagent (Mus musculus) | C57BL6 TMC1 knockout |
MGI: J:184419 | Griffith AJ etc. | From JAX |
| Genetic reagent (Mus musculus) | C57BL6 TMC2 knockout |
MGI: J:184419 | Griffith AJ etc. | From JAX |
| Genetic reagent (Mus musculus) | C57BL6 Lhfpl5 knockout |
MGI: J:98396 | Johnson KR etc. | From JAX |
| Antibody | Chicken anti-GFP | aveslab | RRID:AB_10000240 | Cat:GFP-1020 (1:1000) |
| Antibody | Anti-mouse HA Clone 16B12 | Biolegend | RRID:AB_2565335 | Cat:901513 (1:500) |
| Antibody | Alexa FluroTM 488 goat anti-chicken IgG(H+L) | Invitrogen | RRID:AB_142924 | Cat: A-11039 Lot:1937504 (1:2000) |
| Antibody | Alexa FluroTM 568 goat anti-mouse IgG(H+L) | Invitrogen | RRID:AB_2534072 | Cat: A-11004 Lot:2014175 (1:1000) |
| Sequence-based reagent | TMC1-DF-F | Ruibio Tech |
This paper | 5’:tgagattaacaacaaggaat tcgtgcgtctcaccgttt |
| Sequence-based reagent | TMC1-DF-R | Ruibio Tech |
This paper | 5’:tgagacgcacgaattcctt gttgttaatctcatccatcaaggc |
| Sequence-based reagent | mTMC1-G411C-F | Ruibio Tech |
This paper | 5’: aatgtccctcctgTGTatgtt ctgtcccaccctgtttga |
| Sequence-based reagent | mTMC1-G411C-R | Ruibio Tech |
This paper | 5’:ACAcaggagggacattacc atgttcatttcatttttttcccacca |
| Sequence-based reagent | mTMC1-M412C-F | Ruibio Tech |
This paper |
5’:gtccctcctggggTGTttc tgtcccaccctgtttgactt |
| Sequence-based reagent | mTMC1-M412C-R | Ruibio Tech |
This paper | 5’:ACAccccaggagggacatt accatgttcatttcatttttttccca |
| Sequence-based reagent | mTMC1-N447C-F | Ruibio Tech |
This paper | 5’:tcttcttctaggcTGTttg tatgtattcattctcgcctt |
| Sequence-based reagent | mTMC1-N447C-R | Ruibio Tech |
This paper | 5’:ACAgcctagaagaaga gcaaaaatgcgccccaggag |
| Sequence-based reagent | mTMC1-D528C-F | Ruibio Tech |
This paper | 5’:tctcaccgtttctTGTgtcct gaccacttacgtcacgat |
| Sequence-based reagent | mTMC1-D528C-R | Ruibio Tech |
This paper | 5’:ACAagaaacggtgagacgc acgaattcctgccccaccattgtttc |
| Sequence-based reagent | mTMC1-T532C-F | Ruibio Tech |
This paper | 5’:tgacgtcctgaccTGTta cgtcacgatcctcattggcga |
| Sequence-based reagent | mTMC1-T532C-R | Ruibio Tech |
This paper | 5’:ACAggtcaggacgtcaga aacggtgagacgcacgaattc |
| Sequence-based reagent | mTMC1-D569C-F | Ruibio Tech |
This paper |
5’:atacacagaattcTGT atcagtggcaacgtcctcgctct |
| Sequence-based reagent | mTMC1-D569C-R | Ruibio Tech |
This paper | 5’:ACAgaattctgtgtatgaag gatatccatattctaagtcccagca |
| Chemical compound, drug | Dihydrostreptomycin sulfate | HarveyBio | Cat: HZB1169-1 | |
| Chemical compound, drug | d-Tubocurarine | TCI | Cat: C0433 | |
| Chemical compound, drug | Amiloride | Cayman | Cat: 21069 | |
| Chemical compound, drug | MTSET | Cayman | Cat: 21069 | |
| Chemical compound, drug | GdCl3 | Sigma | Cat: 439770–5G | |
| Chemical compound, drug | LaCl3 | Sigma | Cat: 298182–10G | |
| Chemical compound, drug | CoCl2 | Sigma | Cat: 60818–50G | |
| Chemical compound, drug | ZnCl2 | Sigma | Cat: 793523–100G | |
| Chemical compound, drug | MgCl2 | Sigma | Cat: M8266-100G | |
| Chemical compound, drug |
CaCl2 | Sigma | Cat: 746495–100G | |
| Chemical compound, drug | CsCl | Sigma | Cat:C3139-25G | |
| Chemical compound, drug | KCl | Sigma | Cat:P9333-500G | |
| Chemical compound, drug | NaCl | Sigma | Cat:S7653-1KG | |
| Chemical compound, drug | NaOH | Sigma | Cat:S8045-500G | |
| Chemical compound, drug | KOH | Sigma | Cat:306568–100G | |
| Chemical compound, drug | CsOH | Sigma | Cat:C8518-10G | |
| Chemical compound, drug | BAPTA Tetrasodium salt hydrate | Bioruler | Cat: RH100017-1g | |
| Chemical compound, drug | EGTA | Sigma | Cat: 03780 | |
| Software, algorithm | Igor 6 | WaveMetrics, Inc | ||
| Software, algorithm | Micro-manager 1.4 | micro-manager.org | ||
| Software, algorithm | HEKA patchmaster | HEKA | ||
| Software, algorithm | Matlab 2014 | MathWorks | ||
| Software, algorithm | Prism GraphPad 6 | GraphPad Software. | ||
| Other | HEKA whole cell recording amplifier | HEKA | Order Number: 895273 | |
| Other | Micromanipulator | Sensapex | Cat:uMp-3 |