Figure 4.

Cell-specific gene regulation in P5 Dio3^−/− testis. (A–C) Subsets of 5699 DEGs in Dio3^−/− testis that are specific to different (A) testicular cell types, (B) stages of spermatogenesis, and (C) spermatogonia subtypes, according to the single-cell RNA-sequencing data from Green et al. (47). Green and red dotted lines represent the percentage of upregulated and downregulated genes in all 5699 DEGs genes. ^#P < 0.001, as determined by χ² test on observed vs predicted number of upregulated and downregulated genes. [From: Green CD, Ma Q, Manske GL, Shami AN, Zheng X, Marini S, Moritz L, Sultan C, Gurczynski SJ, Moore BB, Tallquist MD, Li JZ, Hammoud SS. A comprehensive roadmap of murine spermatogenesis defined by single-cell RNA-seq. Developmental Cell 2018;46:651–667.e610.]