Fig. 4.

Comparison of $\text{SCmut}$ to the other methods. (a) Mutations of single cells of the primary tumor of patient BC03 discovered by Mutect. Gray circles are non-mutated sites. The mutated sites are red dots for tumor cells or blue triangles for non-tumor cells. (b) False positive rates computed from $\text{SCmut}$, Mutect, Varscan2 and Monovar. Panels c and d are similar to Panels a and b, respectively, but restricted to cell-level mutations that overlap with mutations from bcWES data. Evaluation of the recovery sensitivity of $\text{SCmut}$ and other methods (Mutect and Monovar) using expected $\text{VAF}$ from bcWES and observed $\text{VAF}$ estimated from single cells are displayed in e and f, respectively