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. 2019 Nov 7;13:1180. doi: 10.3389/fnins.2019.01180

TABLE 2.

PPARα subcellular distribution and cellular localization after incubation with 2-AG and PEA in primary microglial cells.

Mean SEM Mean SEM Mean SEM Mean SEM Mean SEM Mean SEM Mean SEM Mean SEM








Localization CTL PEA 2-AG 2-AG+PEA LPS LPS+PEA LPS+2-AG LPS+2-AG+PEA
1 h
Nucleus 0.53 0.01 0.39 0.01 0.30 0.01 0.34 0.01 0.18 0.01 0.26 0.02 0.65 0.01 0.38 0.01
Cytoplasm 0.04 0.00 0.06 0.00 0.18 0.01 0.03 0.01 0.10 0.00 0.03 0.01 0.03 0.00 0.10 0.00
Both 0.24 0.01 0.26 0.01 0.28 0.01 0.29 0.01 0.36 0.01 0.09 0.01 0.29 0.01 0.14 0.01
No signal 0.20 0.01 0.03 0.00 0.09 0.00 0.03 0.01 0.05 0.00 0.14 0.01 0.01 0.00 0.09 0.00
6 h
Nucleus 0.35 0.01 0.51 0.01 0.22 0.01 0.20 0.01 0.51 0.01 0.53 0.01 0.27 0.01 0.18 0.01
Cytoplasm 0.02 0.00 0.12 0.01 0.16 0.01 0.10 0.01 0.08 0.01 0.06 0.01 0.10 0.01 0.18 0.01
Both 0.63 0.01 0.39 0.01 0.37 0.01 0.45 0.01 0.16 0.01 0.16 0.01 0.31 0.01 0.29 0.01
No signal 0.00 0.00 0.02 0.00 0.08 0.01 0.14 0.01 0.02 0.00 0.12 0.01 0.24 0.01 0.20 0.01
24 h
Nucleus 0.23 0.01 0.50 0.01 0.13 0.01 0.30 0.01 0.30 0.01 0.18 0.01 0.35 0.01 0.23 0.01
Cytoplasm 0.03 0.00 0.03 0.00 0.08 0.01 0.03 0.00 0.03 0.00 0.03 0.01 0.03 0.00 0.00 0.00
Both 0.75 0.01 0.35 0.01 0.95 0.01 0.68 0.01 0.60 0.01 0.55 0.01 0.90 0.01 0.90 0.01
No signal 0.00 0.00 0.10 0.01 0.00 0.00 0.03 0.00 0.15 0.01 0.05 0.01 0.03 0.00 0.00 0.00

Rounded mean values (two positions) and SEM.