Figure 4.

Kinetics of CD137L upregulation; sCD137 suppresses T cell cytokine production only when administered within 24 h of T cell activation. (A) The kinetic profile of Tnfsf9 gene expression in naïve (CD44–) and memory (CD44+) T cells stimulated with CD3/28 beads from three independent experiments. RT-PCR data were normalized based on the expression in each subset at 0 h (baseline). ^*p < 0.05 vs. naïve CD4, ^**p < 0.05 vs. naïve CD8, ANOVA. (B) NOD CD4+ or CD8+ T cells were stimulated with CD3/CD28 dynabeads and cultured with sCD137 added at indicated time points. After a total of 72 h culture, supernatant was collected and IFN-ɤ concentration measured by ELISA. Data expressed as mean and standard errors of mean from three biologically independent experiments and analyzed by ANOVA. ^*p < 0.05, ^**p < 0.01.