Figure 2.

Cigarette smoke-augmented brain infiltration of leukocytes and production of pro-inflammatory factors in MCAO mice. At day 3 after MCAO and reperfusion, brain tissue was harvested from mice receiving cigarette smoke or normal air. Thereafter, single cell suspensions or brain homogenates were prepared from these tissues. (A) Gating strategy for flow cytometry analysis of CD11b⁺CD45^int , CD11b⁻CD45^high, CD11b⁺CD45^high, CD45^highCD11b⁺LY6G⁺, CD45^highCD11b⁺LY6C^high, CD45^highCD3⁺CD4⁺, CD45^highCD3⁺CD8⁺, and CD45^highCD3⁻CD19⁺ cells. (B) Bar graph shows summarized counts of indicated cell subsets. n = 8 per group. Mean ± SD. ^*P < 0.05. (C) Bar graph shows results from mRNA expression levels of cytokines and chemokines in homogenates of the ipsilateral hemisphere brain tissues from indicated groups of mice. n = 6 per group. Mean ± SD, ^*P < 0.05, vs. control group.