Figure 5.

Cigarette smoke induces upregulation of NLRP3 inflammasome in peripheral neutrophils and monocytes. Blood and spleen samples were harvested from groups of mice receiving 4 consecutive days of exposure to cigarette smoke or normal air. (A) Gating strategy for CD11b⁺Ly6G⁺ neutrophils and CD11b⁺Ly6C^high monocytes expressing NLRP3. FMO, fluorescence minus one. (B–D) Quantification of cell counts of the indicated immune cell populations per ml of blood or whole spleen in groups of mice receiving exposure to cigarette smoke or normal air. n = 7 per group. Mean ± SD. ^*P < 0.05.