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. 2019 Nov 13;9:16719. doi: 10.1038/s41598-019-53395-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of vitamin D3 on LPS-stimulated NF-κB and adhesion molecules activation in RCC cells. (A,B) ACHN cells were pretreated with 1,25(OH)2D3, the active form of vitamin D3, and then stimulated with LPS for 6 h. Phosphorylated p65 was measured using Western blot. (A) A representative gel for p-p65 (upper panel) and β-actin (lower panel) was shown. (B) p-p65/β-actin. All experiments were repeated for six times. The grouping of blots cropped from different gels of same samples. All data were expressed as means ± S.E.M. (N = 6). **P < 0.01. (C–E) ACHN cells were pretreated with 1,25(OH)2D3, the active form of vitamin D3, and then stimulated with LPS for 6 h. The level of MCAM, LAMA4 and EpCAM in medium was measured using ELISA. (C) MCAM in medium; (D) LAMA4 in medium; (D) EpCAM in medium. All data were expressed as means ± S.E.M. (N = 8). **P < 0.01.