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. 2019 Nov 13;9:16719. doi: 10.1038/s41598-019-53395-9

Figure 6.

Figure 6

Vitamin D3 reinforces interaction between VDR and NF-κB p65 subunit in RCC cells. ACHN cells were pretreated with 1,25(OH)2D3, the active form of vitamin D3, and then stimulated with LPS for 6 h. The interaction between NF-κB p65 and VDR was detected using CoIP. All experiments were duplicated for four times. A representative gel for NF-κB p65 (upper panel) and VDR (lower panel) was shown. The grouping of blots cropped from different gels of same samples. All data were expressed as means ± S.E.M. (n = 4). **P < 0.01.