Table 1.

Physicochemical characteristics of DFO-NPs

	MW (Da)	# of DFO/NPa	Chelation #b	HD (nm)	KD (M)
DFO	561	1.0c	1.0c	–	4.83 × 10−17
Blank NP	8000	–	–	5.8	4.79 × 10−9
DFO2-NP	9100	1.9	2.0	5.5	1.39 × 10−14
DFO4-NP	10,200	3.6	3.5	5.7	1.37 × 10−17
DFO8-NP	11,300	8.2	7.5	6.5	9.88 × 10−17

MW molecular weight, chelation # chelation stoichiometry, HD hydrodynamic diameter, K_D dissociation constant

^aObtained by ¹H-NMR measurement ^bCalculated by iron binding assay ^cTheoretical value of DFO