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. 2019 Nov 13;9:16720. doi: 10.1038/s41598-019-53325-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sub-nuclear localization of PfHP1 phosphomutants. Representative live cell fluorescence images showing the localization of the GFP-tagged PfHP1–3M and PfHP1-HIM phosphomutants and the PfHP1-Control protein in late schizonts (LS, 40–48 hpi, generation 1; 40 hrs after rapamycin treatment) and after re-invasion in the progeny at late ring stage (LR, 16–24 hpi, generation 2; 64 hrs after rapamycin treatment). Nuclei were stained with Hoechst. DIC, differential interference contrast. Scale bar, 5 µm.