Fig. 6.

Validation of gene expression results by flow cytometry. Flow cytometry MFI for paired lung and LDLN samples from 5 donors is shown for four markers, whose genes were differentially expressed by tissue site by RNA sequencing. P values were calculated using Wilcoxon signed rank test for paired samples. a The expression of CD16, CD74 and CD9 was higher in the lung relative to the LDLN for all CD4 memory T cell subsets. The MFI is shown after gating on all CD4 memory T cells. b The expression levels of CXCR5 and CD200 were higher in the LDLN relative to the lung. CXCR5 gated on all memory CD4 subsets and CD4 TRMs, and for CD200 only in CD4 TRMs (gated on CD4 TRMs). MFI is shown after gating on the cells shown in each panel