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. 2019 Nov 13;9:16686. doi: 10.1038/s41598-019-53032-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Principal Component Analysis (PCA) plot of gene expression patterns in all Calanus spp. samples. The numbers in the symbols indicate the sampling day, i.e. 0, 5, 13 or 20. The borders surrounding the symbols indicate species, i.e. C. finmarchicus (C.fin), C. glacialis (C.gla) or a mix of the two species. C5s in the reference (Ref) group (red, in early diapause) clustered to the far right, and experimental (Exp) adults (green) to the far left. The experimental C5s (blue) were positioned somewhat variably between the reference group and adults, which may suggest some asynchronization in development and diapause phase.