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. 2019 Nov 13;9:16673. doi: 10.1038/s41598-019-53144-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Anesthesia duration and physiology. (a) Anesthesia duration is plotted (dots) separately for bench and fMRI sessions, across all four medetomidine protocols. The bars represent a histogram of anesthesia durations, with all 48 anesthesia sessions binned into one-hour intervals. (b) Heart and respiratory rates (in beats/breaths per minute—bpm), pooled from both bench and fMRI sessions of each medetomidine protocol, are plotted as an across-session mean (solid line) ± s.d. (shaded area). Each of the four medetomidine protocol groups consists of twelve sessions—six animals with two sessions each. The time-points of early wake-ups are indicated with vertical dashed lines. Heart and respiratory rate traces for individual sessions can be found in Supplementary Figs S3 and S4 respectively.