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. 2019 Nov 13;9:16696. doi: 10.1038/s41598-019-53054-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of VEGF supplementation on endothelial progenitor differentiation in male and female cells (A) Representative flow cytometry analysis results showing VEC-GFP expression in H9 (female, left column) and H13 (male, right column) VEC-GFP KI cells after endothelial progenitor differentiation with (bottom row) or without (top row) VEGF (B) Quantification of flow cytometry analysis where each punctum represents an individual experiment (N = 7 for both conditions with H9 and N = 6 for both conditions with H13, ***p < 0.001). (C) Western blot showing the expression of VEC as the cells differentiate with or without VEGF for H9 and H13 cells. β-actin is a housekeeping control.