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. 2019 Nov 13;9:16696. doi: 10.1038/s41598-019-53054-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of endogenous VEGF expression in male and female cells during endothelial differentiation (A) qPCR results from two male cell lines: 6-9-9 (male iPSCs) and 19-9-11 (male iPSCs), and (B) two female cell lines: H9 (female hESCs) and IMR90C4 (female iPSCs), showing expression of VEGF, SOX2, and CD34 over the course of endothelial progenitor differentiation. Student’s T-test was performed to evaluate statistical significance between D0 and D5 expression for VEGF and is indicated on the plot. (ns: not significant, *p < 0.05, ****p < 0.0001).