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. 2019 Nov 14;51(11):135. doi: 10.1038/s12276-019-0342-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Immunoblot analysis for Mob1a/b and p-Yap (S112) in lysates from Mob1a^f/−:Mob1b^f/f:CAGGCre-ER^TM mouse ESCs harvested at the indicated times after 4-OHT treatment. Gapdh served as a loading control. b Immunoblot analysis for Mob1a/b, Oct4, Nanog, and Yap in lysates from Mob1a^f/−:Mob1b^f/f:CAGGCre-ER^TM mESCs harvested at the indicated times after 4-OHT treatment. GAPDH served as a loading control. c Semiquantitative PCR for Mob1a, pluripotency markers (Oct4, Sox2, and Nanog), and differentiation markers (Gata6, Gata4, Sox17, Eomes, T-brachyury, Nestin, and Fgf5) in Mob1a^f/−:Mob1b^f/f:CAGGCre-ER^TM ESCs. d Representative images of alkaline phosphatase staining of Mob1a^f/−:Mob1b^f/f:CAGGCre-ER^TM ESCs on the seventh day after 4-OHT treatment. Scale bars, 200 μm. e Cell cycle analysis of Mob1a^f/−:Mob1b^f/f:CAGGCre-ER^TM mouse ESCs on the seventh day after 4-OHT treatment