Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 13;9:16669. doi: 10.1038/s41598-019-52848-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Quantification of the percentage of arginylated actin in mouse embryonic fibroblasts. (A) Calibration of the antibody signal using purified platelet actin (85% β-actin) and pure N-terminally arginylated β-actin (R-actin) expressed in Pichia pastoris. Error bars represent SEM, n = 6 for R-actin and 5 for platelet actin. The inset in the left graph shows the calibration curve with the additional point to show the limit of the linear range. (B) Quantification of the percentage of R-actin in confluent mouse embryonic fibroblasts (MEF). Error bars represent SEM, n = 5. See also Fig. S1 for antibody specificity and Fig. S2A for the raw data on the antibody signal used for the quantifications in this panel.