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. 2019 Nov 13;9:16669. doi: 10.1038/s41598-019-52848-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Percentage of actin arginylation partially depends on cell confluency. Left, Western blots of MEF cells with different confluency probed with antibodies against R-actin and β-actin as marked. Right top, quantification of total fluorescence from the R-actin band, normalized according to signal of total protein staining. Right bottom, quantification of β-actin and GAPDH in the same samples. Inset on the bottom right shows the representative GAPDH signal used for quantification. Error bars represent SEM, n = 5 for actin and 3 for GAPDH, Student’s t-test was used to determine the P value.