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. 2019 Nov 13;10(11):863. doi: 10.1038/s41419-019-2046-7

Fig. 2. POTEE enhances tumorigenesis and inhibits apoptosis of CRC cells.

Fig. 2

a, b qRT-PCR (a) and western blot (b) were performed to examine POTEE endogenous expression in 11 colon cancer cell lines. c, d Transfection efficiency was detected by qRT-PCR (c) and western blot (d). Results are shown as mean ± SD (n = 3). *P < .05, **P < .01, ***P < .001 based on Student t-test. e MTT (left) and colony formation assays (right) in indicated cell lines with different POTEE expression. Statistics were measured by 2-way ANOVA or Student t, *P < .05, **P < .01, ***P < .001. f, g Migration (f) and invasion (g) assay in indicated cell lines with different POTEE expression. Statistics were measured by Student t, *P < .05, **P < .01, ***P < .001. h Cell-cycle distribution analysis. Results are shown as mean ± SD (n = 3). *P < .05, **P < .01, ***P < .001 based on Student t-test. i Apoptosis analysis with annexin V-APC/PI staining. Cells were exposed to 50 µg/ml 5-Fluorouracil for 36–48 h. Results are shown as mean ± SD (n = 3). *P < .05, **P < .01, ***P < .001 based on Student t-test